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In vivo hairpin extrusion. (A) Primer extension analysis of RNAs synthesized in E. coli cells from promoters P1 or P1 G-13 and the respective P2 control promoters. P1 and P1 G-13 promoter template-strand sequences are shown. Short arrow, primer extension product. (B) Primer extension analysis of RNAs synthesized in E. coli cells from rrnB P1 wild-type or mutant promoters containing extruding and nonextruding hairpin sequences between the −10 and −35 hexamers.
