Table 1.

T_m of template- and non-template-strand hairpins and the effect of mutations

Promoter	Sequence	Tm, °C
P1	5′-AGTTGC-G−11 A A−13-GCAACG-3′	76.7 ± 0.5
	G-    -C	69.8 ± 0.2
	C	69.4 ± 0.6
	T	66.0 ± 0.6
	C         G	75.6 ± 0.3
	A   G	68.5 ± 0.3
	T-    -A	65.5 ± 0.4
	A-    -T	63.6 ± 0.2
	G	74.9 ± 1.9
	T	75.7 ± 0.3
	C	76.7 ± 0.3
	5′-CGTTGC-T T C-GCAACT-3′	67.5 ± 0.5
P2	5′-AGAAGC-G G A-GCTTCT-3′	73.6 ± 1.0
	G-     -C	61.2 ± 0.7
	C	67.5 ± 1.0
	T	63.6 ± 1.1
	C         G	73.9 ± 0.5
	T	72.0 ± 0.4
	C	74.9 ± 0.2
	A	75.0 ± 0.4
	5′-AGAAGC-T C C-GCTTCT-3′	69.4 ± 0.6
P3	5′-AGCTGC-G G A-GCAGCT-3′	84.0 ± 2.0
	5′-AGCTGC-T C C-GCAGCT-3′	76.3 ± 0.6

Numbering of sequence on template strand corresponds to numbering of promoter template strand.