Figure 1.
Effect of chaperonins on the surface pressure of lipid monolayers. GroEL14 or GroES7 oligomers were injected underneath the monolayer and the surface pressure was measured. (A) At time points indicated by arrows, 3.5 μM GroEL, 1.5 mM ATP, and 7 μM GroES were added. (B) The same as in A, with reverse order of events. (C) Surface pressure increases as a function of GroEL concentration in the subphase. Different amounts of GroEL (—), GroES (- - -), or MDH (– – –) were injected into the subphase. The increase in surface pressure was measured after pressure equilibration.