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Effect of pH on GroEL–monolayer interaction. (A) At the indicated time, 3.5 μM GroEL was added to the subphase. After pressure equilibration, the pH of the subphase was lowered to 4 with HCl. Where indicated, the pH was readjusted to 7.5 with NaOH. (B) pH-dependent surface pressure increase caused by the addition of 0.35 μM GroEL.
