Table 2.
Effect of anti-angiogenin monoclonal antibody on the activity of angiogenin in HUVE cells
| Samples | [3H]thymidine incorporation, cpm (% increase) | Proliferation, no. of cells (% increase) |
|---|---|---|
| Control | 11,700 ± 1,300 (100) | 14,800 ± 800 (100) |
| Angiogenin | 15,600 ± 1,100 (133) | 17,200 ± 300 (116) |
| 26-2F | 11,700 ± 1,500 (100) | 14,900 ± 200 (101) |
| Angiogen plus 26-2F | 11,500 ± 1,200 (98) | 15,100 ± 1,000 (102) |
| VEGF | 17,300 ± 600 (148) | 35,300 ± 1,000 (239) |
| VEGF plus 26-2F | 17,900 ± 400 (153) | 32,500 ± 1,200 (220) |
| bFGF | 95,300 ± 500 (815) | 32,900 ± 300 (222) |
| bFGF plus 26-2F | 95,600 ± 800 (817) | 29,800 ± 300 (201) |
HUVE cells were plated at 15,000 cells per 35-mm culture dish (1,500 cells per cm2). Concentrations of angiogenin, VEGF, FGF, and 26-2F were 20 ng/ml, 5 ng/ml, 20 ng/ml, and 10 μg/ml, respectively.