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. 1997 Mar 18;94(6):2215–2220. doi: 10.1073/pnas.94.6.2215

Figure 3.

Figure 3

Competitive reconstitution of UV-damaged DNA. Comparison of the effect of different levels of UV damage on 214-bp 5S DNA reconstitution. Lanes: 1–3, free DNA:core particle ratio of 0.5:1; 4–6, free DNA:core particle ratio of 1:1; 7–9, free DNA:core particle ratio of 2:1. UV dose levels are indicated above each lane. Rec., approximate area used to calculate reconstituted fraction of 5S DNA; Free DNA, approximate area used to calculate DNA fraction of 5S DNA. Multiple bands in the reconstituted region on these gels are due (at least in part) to multiple translational positions of the nucleosome on the 5S DNA.