Figure 2.

Radiochromatograms of zinc exchange between ⁶⁵Zn₇-MT-1 and Zn₇-MT-2. Proteins (10.3 μM total MT) were incubated, an aliquot was loaded at the times indicated on a DEAE MemSep-1000 cartridge at a flow rate of 9.6 ml/min, the cartridge was eluted in 10 min with a linear gradient from 0 to 10 mM sodium chloride in 10 mM Tris·Cl (pH 8.6), and the radioactivity in the fractions was measured. Total counts injected were 16,409 cpm for MT-1 and 21,364 cpm for the reaction mixture.