Table 2.

Kinetics of flap mutants

Enzyme	Substrate
	KARVL/AEAMSF (NO2)			KAVY/F (NO2) EANle-NH2
	Km, mM	kcat, s−1	kcat/Km	Km, μM	kcat, s−1	kcat/Km
Wild-type	0.49	8.5	17.3	3.7	13.4	3.6
Ile-50 → Val	3.13	8.3	2.7	16.4	19.5	1.2
Gly-51 → Ala	0.73	1.5	2.1	2.2	2.6	1.2
Phe-53 → Asp	0.21	3.9	18.6	6.9	15.0	2.2
Ile-54 → Thr	>5	ND	ND	20.4	1.5	0.1

ND, not determined. One-letter abbreviations for amino acids are used.