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Purification of CH from H. virescens
| Fraction | Total activity, milliunits | Protein, mg | Specific activity, milliunits/mg | Recovery, % |
|---|---|---|---|---|
| Larval gut extract | 690 | 187 | 3.7 | |
| Ammonium sulfate precipitation (52–65%) | 505 | 19 | 26.6 | 73 |
| Acetone fractionation (44.5–55.4%) | 385 | 4.8 | 80.2 | 56 |
| G-200 Sephadex chromatography | 115 | 0.8 | 143.7 | 17 |
Experimental details are provided in the text.
