Table 2.
Types of mutations in spontaneous and UV-induced (300 J/m2) pLS76 and in human cells
| Sequence alteration | No. of mutations (% of total)
|
||
|---|---|---|---|
| Untreated* | UV-induced* | Human skin p53† | |
| Transitions | |||
| GC to AT | 5 (21) | 38 (61) | 105 (45) |
| AT to GC | 1 (4) | 3 (5) | 18 (8) |
| Transversions | |||
| GC to TA | 4 (17) | 7 (11) | 22 (9) |
| GC to CG | 0 (0) | 3 (5) | 14 (6) |
| AT to TA | 0 (0) | 0 (0) | 9 (4) |
| AT to CG | 2 (8) | 0 (0) | 10 (4) |
| CC to TT (or GG to AA) | 0 (0) | 4 (6) | 32 (14) |
| Other double mutants | 0 (0) | 2 (3) | 5 (2) |
| Deletion (<10 bp) | 3 (12) | 3 (5) | 12 (5) |
| Deletion (>10 bp) | 5 (21) | 1 (2) | 2 (1) |
| Insertion (<10 bp) | 0 (0) | 1 (2) | 4 (2) |
| Insertion (>10 bp) | 4 (17) | 0 (0) | 0 (0) |
| Total mutations | 24 | 62 | 233 |
*
Mutations in exons 5–8 of p53 in pLS76 (this study).
†
Noncryptic mutations in the coding region of p53 in human skin tumors, precancerous lesions, and cell lines (13).