Figure 1.

Nucleotide sequence of early H2A gene and flanking regions. Thick underlines indicate the binding sites for the MBF-1 and CCAAT binding factor (CBF) factors to, respectively, the modulator and the CCAAT sequences of the H2A promoter. Arrow refers to the transcription starting site. The deduced amino acid sequence of the H2A coding region is reported above the DNA sequence. Arrowheads pointing to the sequence refer to the mesenchyme blastula-specific micrococcal nuclease (MNase) cutting sites. The 3′ palindrome is indicated by arrows while the conserved motif for the RNA processing is overlined. The sns sequence is boxed, and the HpaII and Sau3A recognition sites flanking the sns fragment are underlined.