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Solubility analysis and purification of singly and coexpressed ECRL and USPL (a) and coexpressed ECRL and RXRL (b). ECRL was fused with GST. USPL and RXRL were tagged with histidine. The total cell lysates (Total), the soluble (Soluble) and insoluble (Insoluble) fractions, and the purified protein bound on the Ni2+ NTA beads (Bound) are indicated above each lane.
