Figure 1.

Schematic representation of plasmids used in the two-hybrid system pLexA-IR-S and pLexA-IR-L are the bait fusion plasmids containing the short or long fragment of the insulin receptor β subunit, respectively. pJG4–5-cDNA is the cDNA library derived from HeLa cells mRNAs. pSH18–34 is the reporter plasmid. EC, extracellular; TM, transmembrane domain; PTK, protein tyrosine kinase; MCS, multiple cloning sites; NLS, nuclear localization signal; AD, activation domain; HA, hemagglutinin tag sequence; LexA BS, LexA binding site.