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Effect of storage on recovery of PCR-amplifiable DNA from supernatant fractions. The number of supernatant PCR positive samples in fresh and stored samples is plotted for all samples (n = 87), initially 3+ (n = 55), 2+ (n = 23) and 1+ (n-9) samples. The increase in supernatant PCR positivity upon storage was statistically significant (P < 0.0001 among 3+ and 2+ samples and P = 0.012 in 1+ samples).
