Specific detection of phospholipid classes in an unprocessed lipid extract from CHO cells by negative ion ESI tandem MS. (a) Detection of PI by precursor ion scanning for m/z 241 (offset, +45 V). (b) Detection of PS by scanning for neutral loss of 87 D (collision cell offset, +25 V). (c) Detection of PE by precursor ion scanning for m/z 196 (collision cell offset, +40 V). (d) Detection of SM by combined application of skimmer-CID and parent ion scanning for m/z 168 (sCID offset, +65 V; collision cell offset, +40 V).