Figure 5.

Specific detection of phospholipid classes in an unprocessed total lipid extract of CHO cells by positive ion ESI-MS/MS. (a) Detection of [M+H]⁺ ions of PC and SM by precursor scanning for m/z 184 (collision cell offset, −35 V). (b) Detection of [M+H]⁺ ions of PE by scanning for neutral loss of 141 D (collision cell offset, −25 V). (c) Detection of [M+H]⁺ ions of PS by scanning for neutral loss of 185 D (collision cell offset, −22 V).