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An unprocessed total lipid extract of 5000 CHO cells containing equimolar amounts of PC-(24:0), -(28:0), -(40:0), and -(44:0) was analyzed by parent ion scanning for m/z 184. (a) Uncorrected ion intensities. The signal intensities of the internal standards were used for generation of the calibration plot insert. (b) Corrected ion intensities of the PC signals so that the monoisotopic signals represent the true molar abundances of the corresponding PC molecular species.
