Abstract
Serum from rats bearing progressively growing aminoazo dye-induced rat hepatomata has been fractionated by Sephadex G150 gel filtration chromatography and isolated fractions have been examined by indirect membrane immunofluorescence techniques to detect tumour specific antigen and antibody. Hepatoma D23-specific antigenic activity was associated with material (of approximate molecular weight <150,000) isolated in the included volume of the gel at pH 7·3. The fraction excluded from the gel (of approximate molecular weight >150,000) was adjusted to pH 3·0 and further separated by Sephadex G150 gel filtration chromatography at pH 3·0 into gel included and excluded fractions. Hepatoma D23 specific antibody, demonstrable by membrane immunofluorescence staining of hepatoma D23 cells, was found to be eluted in the excluded volume and specific antigenic activity was retarded into the included volume of the gel. These results indicate that hepatoma D23 bearer serum contains free circulating tumour specific antigen in excess, together with specific immune complexes. The presence of these factors in tumour bearer serum is discussed in terms of “blocking” phenomena whereby serum factors may protect tumour cells from sensitized lymphocyte cytotoxic attack.
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Selected References
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- Baldwin R. W., Barker C. R. Demonstration of tumour-specific humoral antibody against aminoazo dye-induced rat hepatomata. Br J Cancer. 1967 Dec;21(4):793–800. doi: 10.1038/bjc.1967.93. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Baldwin R. W., Embleton M. J., Robins R. A. Cellular and humoral immunity to rat hepatoma-specific antigens correlated with tumour status. Int J Cancer. 1973 Jan 15;11(1):1–10. doi: 10.1002/ijc.2910110102. [DOI] [PubMed] [Google Scholar]
- Baldwin R. W., Glaves D. Solubilization of tumour-specific antigen from plasma membrane of an aminoazo-dye-induced rat hepatoma. Clin Exp Immunol. 1972 May;11(1):51–56. [PMC free article] [PubMed] [Google Scholar]
- Baldwin R. W., Harris J. R., Price M. R. Fractionation of plasma membrane-associated tumor-specific antigen from an aminoazo dye-induced rat hepatoma. Int J Cancer. 1973 Mar 15;11(2):385–397. doi: 10.1002/ijc.2910110216. [DOI] [PubMed] [Google Scholar]
- Baldwin R. W., Price M. R., Robins R. A. Blocking of lymphocyte-mediated cytotoxicity for rat hepatoma cells by tumour-specific antigen-antibody complexes. Nat New Biol. 1972 Aug 9;238(84):185–186. doi: 10.1038/newbio238185a0. [DOI] [PubMed] [Google Scholar]
- Currie G. A., Basham C. Serum mediated inhibition of the immunological reactions of the patient to his own tumour: a possible role for circulating antigen. Br J Cancer. 1972 Dec;26(6):427–438. doi: 10.1038/bjc.1972.59. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hellström I., Hellström K. E. Studies on cellular immunity and its serum mediated inhibition in Moloney-virus-induced mouse sarcomas. Int J Cancer. 1969 Sep 15;4(5):587–600. doi: 10.1002/ijc.2910040503. [DOI] [PubMed] [Google Scholar]
- Sjögren H. O., Hellström I., Bansal S. C., Hellström K. E. Suggestive evidence that the "blocking antibodies" of tumor-bearing individuals may be antigen--antibody complexes. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1372–1375. doi: 10.1073/pnas.68.6.1372. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sjögren H. O., Hellström I., Bansal S. C., Warner G. A., Hellström K. E. Elution of "blocking factors" from human tumors, capable of abrogating tumor-cell destruction by specifically immune lymphocytes. Int J Cancer. 1972 Mar 15;9(2):274–283. doi: 10.1002/ijc.2910090205. [DOI] [PubMed] [Google Scholar]
- Thomson D. M., Steele K., Alexander P. The presence of tumour-specific membrane antigen in the serum of rats with chemically induced sarcomata. Br J Cancer. 1973 Jan;27(1):27–34. doi: 10.1038/bjc.1973.4. [DOI] [PMC free article] [PubMed] [Google Scholar]