Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
RT-PCR analysis for exogenous and endogenous MDC mRNA expression in genetically modified DC. Total cellular RNA (1 μg) from DCs transduced with AdMDC, AdNull, or PBS alone (naive control) were reverse transcribed using the oligo(dT)18 primer. Serial 1:10 dilution of RT-generated cDNA were used as a template to amplify exogenous MDC, endogenous MDC, and GAPDH cDNA fragments by PCR. Samples were separated by 1.5% agarose gel electrophoresis and stained with ethidium bromide.
