Figure 3.

Chemoattracting activity of AdMDC-modified DCs for CCR4-expressing cells in vivo. To demonstrate the in vivo function of AdMDC-modified DCs, C57BL/6 mice were intravenously injected with AdMDC- or AdNull-modified DCs. Genetically modified DCs were labeled with PKH26 (red fluorescence) before the injection for in vivo cell tracking. After 4 days, spleens were dissected, and frozen spleen sections were stained using rabbit anti-mouse CCR4 Ab followed by visualization with anti-rabbit IgG Ab labeled with FITC (green fluorescence). Nuclei were stained with DAPI (blue fluorescence).