Figure 6.
In vivo Pseudomonas-specific Ab responses of mice immunized with AdMDC-modified DCs pulsed with Pseudomonas. (a–f) Titer of anti-Pseudomonas Ab in serum. C57BL/6 mice were immunized with heat-killed P. aeruginosa-pulsed (PAO1 strain) and AdMDC-modified (or AdNull-modified) DCs. Two weeks after immunization, each isotype of anti-Pseudomonas (PAO1 strain) Ab was assessed in serum using a standard ELISA protocol. Data are presented as mean ± SE (n = 3 mice per data point) of end-point titers. (a) IgM, (b) IgG1, (c) IgG2a, (d) IgG2b, (e) IgG3, (f) IgA. (g) Passive transfer of serum. Three weeks after immunization, serum was collected and pooled from C57BL/6 mice immunized as in a–f. Each recipient C57BL/6 mouse received 100 μl of heat-inactivated (at 56°C for 30 minutes) serum intravenously. After 6 hours, recipient animals were challenged by intratracheal injection with 2 × 105 CFU P. aeruginosa strain PAO1 enmeshed in agar beads (day 0). Controls included mice without any infusion of serum. Survival was recorded as the percentage of surviving animals (n = 10 mice per group).