Figure 10.

Western blot analysis of fibroblast culture ECM. (a) Intact collagen type-I (upper doublet; 120 and 125 kDa) was reduced TIMP-3–null fibroblast culture ECM with a corresponding appearance of an approximately 90-kDa degradation fragment (three-quarter fragment). Collagen type-I degradation was partially rescued by the addition of GM6601 to the null culture. (b) Intact collagen type-IV abundance was reduced in the ECM collected from null fibroblasts compared with control cells. Addition of GM6001 to inhibit MMPs in the null cultures partially rescued the degradation. (c) Silver-stained SDS-PAGE gel demonstrating a global reduction in protein abundance in null ECM again partially reversible by the addition of GM6001 to null cultures.