Figure 5.

Effect of dominant-negative human UbcH10 on cell cycle progression in mammalian somatic tissue culture cells. (A) COS cells were mock-transfected (control) or transfected with AU1-tagged wild-type (WT) or dominant-negative (mutant) UbcH10, and after 48 h were fixed and stained with AU1 antibodies and Hoechst 33342.Two hundred cells each were scored for the percent cells in interphase vs. M phase. (B) Immunoblots of total protein extracts from cells transfected with either wild-type or dominant-negative AU1-tagged UbcH10, reacted with cyclin A or B antibodies. (C) Morphologies of cells transfected with either wild-type (Left) or dominant-negative UbcH10 (Right). Cells were stained with AU1 antibodies (Top) to identify transfected cells, and with Hoechst dye (Bottom) to monitor chromosomes.