Figure 6.

Hoechst-stained fields of cells from embryos injected with RNA encoding wild-type (striped bars) or dominant-negative mutant (black bars) clam E2-C. Fertilized Xenopus eggs were injected with 10 ng RNA encoding wild-type or dominant-negative clam E2-C and cultured to late blastula stage. Cells were fixed and stained with Hoechst 33342. (A) Two hundred cells in M phase were scored for percent in prophase, metaphase, and anaphase/telophase. (B) A field of Hoechst-stained chromosomes from embryos injected with wild-type (Left) and dominant-negative mutant (Right) RNA.