Figure 3.
Combination therapy with GM-CSF and IL-2, but not IL-2 alone, induces expansion of EBV-specific T cells in vivo. Flow cytometric analysis of splenocytes from SCID mice engrafted with PBMCS from an EBV-seropositive donor (HLA A1/A1, B8/B8), and treated weekly with ASGM-1 and daily with either IL-2 alone (upper panels) or GM-CSF and IL-2 (lower panels). HLA tetramers complexed with immunodominant (a) lytic (RAK) or (b) latent (FLR) EBV peptides were used to identify expanded populations of human CD3+ subsets with EBV-specificity.