Northern blot analysis of bovine skin RNA using
pNPI specific probes. Twenty micrograms of poly(A)+ RNA
(lanes A and B) or 40 μg of total RNA (lanes C and D) were separated
by electrophoresis in agarose/formaldehyde gel, transferred onto a
nylon membrane, and hybridized with pNPI-specific probes (lanes A and
B, OP1 and OP10 oligonucleotide probes, respectively; lanes C and D,
cDNA probes from clones 12 and 41). The membranes were exposed to x-ray
film. Positions of 28S and 18S ribosomal RNA are indicated.