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. 2001 Oct 1;108(7):1051–1059. doi: 10.1172/JCI12467

Figure 5.

Figure 5

(a) SLO-permeabilized BON cells. Cells were permeabilized by 20 U/ml SLO for 5 minutes in permeabilizing buffer and preincubated with 200 μM of GDP-β-S for 5 minutes before stimulation (shaking at 80 rpm): 0 rpm, 2.9 ± 0.4 pmol/well/20 min; 0 rpm + GDP-β-S, 3.1 ± 0.3 pmol/well/20 min. *P < 0.05 versus permeabilized static control, +P < 0.05 versus shaking at 80 rpm of permeabilized cells (n = 9). (b) BON cells were permeabilized by 20 U/ml SLO for 5 minutes in permeabilizing buffer and preincubated with 25 μg/ml of NH2-terminal peptide (GqN17) or COOH-terminal peptide (GqC20) for 5 minutes before mechanical stimulation at 80 rpm. Experiments were performed in the presence of 1× protease inhibitor cocktail. 5-HT release in pmol/well/20 min was 0 rpm, control: 2.2 ± 0.02; GqN17: 1.8 ± 0.4; GqC20: 2.1 ± 0.07. *P < 0.05 versus permeabilized static control, +P < 0.05 versus shaking at 80 rpm of permeabilized cells (n = 3).