Figure 1.

Kinetics of HBsAg synthesis and secretion in glucosidase-inhibited cells. After 3 h in the absence or presence of glucosidase inhibitor (NB-DNJ), 2.15 cells were pulse labeled for 20 min with [³⁵S]methionine and chased with medium lacking radioactivity. At the indicated times after labeling, the amount of HBsAg in cell lysates (cells) and culture medium (medium) was determined by the antigen capture assay described in Materials and Methods using mAb C20-02, which recognize L, M, and S. The amount of HBsAg bound to plates coated with antibody is presented as cpm.