Figure 2.

Phosphorylation of nuclear annexin II and cell cycle distribution. Human K562 cells were subjected to centrifugal elutriation followed by flow cytometry to determine the cell cycle distribution of phosphorylated annexin II. Cells enriched in each indicated phase were fractionated to cytosolic extract (CE) and nuclear extract (NE). Twenty μg protein from each sample were subjected to SDS-PAGE followed by immunoblotting with anti-annexin II antibody. Immunoblot of PGK was used as an internal control. The flow cytometric profile of each fraction is presented in the upper panels and the percentage of cells in each cell cycle phase is indicated below the immunoblot under each fraction.