Figure 5.

Localization of wild type and NES mutant annexin II in transiently transfected LNCaP cells. Transiently transfected LNCaP cells expressing GFP alone, GFP-wild type (GFP-WT) annexin II or GFP-NES mutant (GFP-NES) annexin II were fixed and subjected to immunocytochemistry with anti-annexin II antibody as described in materials and methods. The images were scanned using laser scanning confocal microscope (LSCM). The green color represents auto-fluorescence of GFP, the red color represents the rhodamine staining of annexin II antigen-antibody complex, and the yellow color is the overlay of GFP and annexin II images. Bar: 25 μm.