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Reelin is a substrate for plasmin. Recombinant reelin was digested with plasmin (Sigma, from human plasma, 0.5 unit/ml) for various times, loaded in duplicate and immunoblotted. 1 no incubation, 2 10 min, 3 30 min, 4 6 h, 5 digested for 6 h in the presence of aprotinin (Sigma, 40 μM).
