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. 1997 Mar 18;94(6):2386–2391. doi: 10.1073/pnas.94.6.2386

Figure 2.

Figure 2

ML.ΔβRII gross phenotype, histopathology, BrdUrd labeling, and K6 expression. (A) A transgenic pup (Upper) from line B9223 shows a thickened and wrinkled skin compared with the nontransgenic sibling (Lower) 24 hr after birth. (B) Transgenic mice (Left and Right) from line B9223 exhibited a peeling skin phenotype 3 days after birth. (C–F) Richardson’s staining of 1 μm sections. (×20.) (C) Nontransgenic skin 24 hr after birth. (D) ML.ΔβRII transgenic skin from line B9223 shows a 3-fold increase in thickness of the epidermis, hyperkeratosis, and hypertrophic keratinocytes compared with the control (C). (E) Nontransgenic skin 3 days after birth. (F) ML.ΔβRII transgenic skin from line B9223 shows a moderate hyperplasia at 3 days, and a more loosely attached stratum corneum compared with the transgenic skin at 24 hr (D). (G–J) Double-label immunofluorescence. (×20.) (Red represents K14.) (G) BrdUrd labeling (green or yellow) in a nontransgenic epidermis. (H) ML.ΔβRII transgenic epidermis exhibits about a 3-fold increase in BrdUrd labeling. (I) K6 is only expressed in the hair follicles of the normal skin (green or yellow). (J) K6 overexpression in ML.ΔβRII transgenic epidermis.