Figure 3.
Analysis of ML.ΔβRII keratinocytes in culture. (A) Nontransgenic keratinocytes were subconfluent at day 4 of culture. (B) Keratinocytes from ML.ΔβRII epidermis grew faster and were confluent by day 4. (C) [3H]TdR labeling of keratinocytes 22 hr after addition of TGF-β1. [3H]TdR labeling for cultures receiving different concentrations of TGF-β1 was expressed as percent of control. Each point is the average of results determined in triplicate. Error bars represent SD. Note that nontransgenic keratinocytes showed a marked decrease in [3H]TdR labeling at the lowest concentration of TGF-β1, while ML.ΔβRII keratinocytes were resistant to TGF-β1 until the concentration reached 0.5 ng/ml.