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. 2001 Dec 1;108(11):1667–1675. doi: 10.1172/JCI13202

Figure 5.

Figure 5

Activation of NF-κB pathway in B cells from PPARγ+/– mice. (a) Nuclear extracts from B cells were analyzed by EMSA for the ability to bind to an NF-κB consensus probe. Lane 1: No extract, with labeled oligonucleotide; lane 2: resting B cells from wild-type mice (+/+); lane 3: resting B cells from PPARγ+/– mice (+/–); lane 4: stimulated B cells by LPS for 5 minutes from wild-type mice; lane 5: stimulated B cells from PPARγ+/– mice; lane 6: lane 5 with excess of cold oligonucleotide; lane 7: lane 5 without labeled oligonucleotide. (b) Phosphorylation of IκBα. Cell lysates from B cells were probed with anti-phosphorylated IκBα (lanes 1–4) and anti- IκBα (lanes 5–8). Lanes 1 and 5 were resting B cells from wild-type mice. Lanes 2 and 6 were resting B cells from PPARγ+/– mice. Lanes 3 and lane 7 were B cells from wild-type mice, stimulated with LPS for 5 minutes. Lanes 4 and 8 were stimulated B cells from PPARγ+/– mice.