Figure 2.

Analysis of NLOs in CDG patients and a control subject. Metabolically labeled NLOs were isolated from a healthy individual (control subject) and from patients A, L, and S (CDG-If patient A, CDG-If patient L, CDG-If patient S). [³H]-labeled NLOs were cleaved from protein by incubation with PNGaseF, protein was removed by precipitation, and the liberated oligosaccharides were analyzed by HPLC using a radiodetector to monitor elution positions. Elution positions for standard oligosaccharide intermediates Man₃GlcNAc₂ to Glc₁Man₉GlcNAc₂ (M₃, M₄, M₅, M₆, M₇, M₈, M₉, G₁M₉) are given at the top of the figure.