Figure 4.

Alignment of the primary sequence of Lec35 proteins from human (Homo sapiens, GenBank accession no. XP_008236), mouse (Mus musculus, accession number Q9R0Q9), hamster (Cricetulus griseus, accession no. Q60441), worm (Caenorhabditis elegans, accession no. AAA83473), and plant (Arabidopsis thaliana, assembled from genomic clone AF147262). Regions conserved in the five proteins are shaded in black, and residues that are conserved in four proteins are shaded in gray. The amino acid substitutions and the change of reading frame detected in CDG-If patients are indicated above the human sequence. The line following the Δ-mark for the mutation 511delC shows the portion of the polypeptide translated in another reading frame before a stop codon appears (vertical line).