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Sequence-specific DNA binding of GST-Ci. P, free BS-1 DNA; C, GST-Ci/BS-1 complex; S, supershifted complex. Lane 1, BS-1 (TGGGTGGTC) alone. Lanes 2–5, GST-Ci/BS-1 complex with 0, 10, 100, and 500 ng competing unlabeled BS-1. Lanes 6–9, GST-Ci/BS-1 complex with 0, 10, 100, and 500 ng competing unlabeled BS-2 (TGGGTAGTC). Lanes 10 and 11, GST-Ci/BS-1 complex supershifted with 1 and 2 μl GST antiserum.
