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Northern blot analysis indicates that CRM3 is expressed during sporophytic and gametophytic phases of the fern life cycle. Note that the autoradiography showing CRM3 expression was exposed ≈5 times longer than the GAPA loading control. RNA sources were gametophytes (Ga) harvested 11 days after spore soaking or fertile fronds of the sporophyte (Sp) as indicated. CRM1 expression was found to be very similar to that of CRM3, albeit weaker (data not shown).
