Abstract
An enzyme-linked immunosorbent assay (ELISA) was developed in which a tumour-specific component of human squamous-cell carcinoma of the lung could be readily detected using an absorbed rabbit antiserum. This antiserum did not react with equivalent preparations made from pooled normal lung tissue. In a study using the coded sera from normal individuals and preoperative patients subsequently shown to have Stage I bronchogenic carcinoma of various histological types, we found that the patients' sera effectively inhibited the reaction between the rabbit antiserum and the partially purified tumour antigen, whereas the serum from normal individuals did not.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Braatz J. A., McIntire K. R., Princler G. L., Kortright K. H., Herberman R. B. Purification and characterization of a human lung tumor-associated antigen. J Natl Cancer Inst. 1978 Oct;61(4):1035–1046. [PubMed] [Google Scholar]
- Kelly B. S., Levy J. G. Purification of a protein associated with human bronchogenic squamous-cell carcinoma. Br J Cancer. 1979 Mar;39(3):224–233. doi: 10.1038/bjc.1979.45. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kelly B., Levy J. G. Evidence for a common tumour-associated antigen in extracts of human bronchogenic carcinoma. Br J Cancer. 1977 Jun;35(6):828–833. doi: 10.1038/bjc.1977.125. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Porath J., Axen R., Ernback S. Chemical coupling of proteins to agarose. Nature. 1967 Sep 30;215(5109):1491–1492. doi: 10.1038/2151491a0. [DOI] [PubMed] [Google Scholar]
- Watson R. D., Smith A. G., Levy J. G. The detection by immunodiffusion of tumour associated antigenic components in extracts of human bronchongenic carcinoma. Br J Cancer. 1975 Sep;32(3):300–309. doi: 10.1038/bjc.1975.228. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yachi A., Matsuura Y., Carpenter C. M., Hyde L. Immunochemical studies on human lung cancer antigens soluble in 50 per cent saturated ammonium sulfate. J Natl Cancer Inst. 1968 Apr;40(4):663–682. [PubMed] [Google Scholar]