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Confirmation of secretion of SER by S. aureus isolates by Western blot analysis. Using anti-rSER antibody, we examined SER productivity of S. aureus isolates from a food poisoning outbreak in Fukuoka. Culture supernatants from Fukuoka 5 (F5), Fukuoka 6 (F6), Fukuoka 7 (F7), and Fukuoka 8 (F8) were subjected to Western blot analysis. An approximately 30-kDa signal was detected by anti-rSER antibody. P1, 25 ng of rSER per well; P2, 50 ng of rSER per well.
