FIG. 1.

Representative dot blot hybridization of SSH products. Cloned SSH products were PCR amplified and probed with ³²P randomly labeled genomic DNAs of S. suis serotype 2 strains 89-999 (virulent) and 90-1330 (avirulent). A, E, and R, clones from AluI-, EcoRV-, and RsaI-subtracted libraries, respectively; pKS1, -2, -4, and -6, RsaI-subtracted fragments cloned in pBluescript II KS; 999, DNA from virulent Canadian strain 89-999; 1330, DNA from avirulent Canadian strain 90-1330; S-735, DNA from virulent European strain S-735. Circles indicate the PCR products belonging to phage sequences.