FIG. 2.

An example of the screening process for the confirmation that candidate gene products react specifically with the pooled convalescent-phase serum. Reactive clones on the primary screening plate (A) were picked up, transferred to a 96-well culture plate, and replica plated onto a IPTG-KAN-BHI agar plate for the secondary screening (B). Serum from a 13-year-old girl who had never eaten raw shellfish was adsorbed with in vitro-expressed antigens of V. vulnificus and used as a negative control. (C) Each clone was tested in duplicate or triplicate. NEG and CON, negative and pooled convalescent-phase sera, respectively. Arrows indicate positive clones.