TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Source or reference |
|---|---|---|
| Strains | ||
| V. vulnificus | ||
| MO6-24/O | Clinical isolate | 47 |
| CMCP6 | Clinical isolate | Chonnam National University Hospital |
| CMCP98K | Clinical isolate | Chonnam National University Hospital |
| CMM1701 | CMCP6 with the insertion mutation in Vv-ive-1 gene; Cmr | This study |
| CMM1702 | CMCP6 with the insertion mutation in Vv-ive-2 gene; Cmr | This study |
| CMM1703 | CMCP6 with the insertion mutation in Vv-ive-3 gene; Cmr | This study |
| CMM1704 | MO6-24/O with the insertion mutation in Vv-ive-4 gene; Cmr | This study |
| CMM1705 | CMCP6 with the insertion mutation in Vv-ive-5 gene; Cmr | This study |
| CMM1706 | CMCP6 with the insertion mutation in Vv-ive-6 gene; Cmr | This study |
| CMM1707 | CMCP6 with the deletion mutation in Vv-ive-7 gene | This study |
| CMM1708 | MO6-24/O with the deletion mutation in Vv-ive-8 gene | This study |
| CMM1709 | MO6-24/O with the insertion mutation in Vv-ive-9 gene; Cmr | This study |
| CMM1710 | CMCP6 with the deletion mutation in Vv-ive-10 gene | This study |
| CMM1711 | CMCP6 with the insertion mutation in Vv-ive-11 gene; Cmr | This study |
| CMM1712 | CMCP6 with the insertion mutation in Vv-ive-12 gene; Cmr | This study |
| CMM1715 | CMM1710 containing pCMM1701; Ampr Tcr | This study |
| E. coli | ||
| BL21(DE3) | F−ompT hsdSB(rB− mB−) gal dcm (DE3) | Novagen |
| DH5α | F− φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK− mK+) phoA supE44 λ−thi-1 gyrA96 relA1 | Laboratory collection |
| SY327 λpir | Δ(lac pro) argE(Am) rif nalA recA56 λpir lysogen | 39 |
| SM10 λpir | thi thr leu tonA lacY supE recA::RP4-2-Tcr::Mu λpirR6K lysogen; Kmr | 39 |
| Plasmids | ||
| pET30a,b,c | Inducible-expression vectors carrying N-terminal His · Tag/thrombin/S · Tag/enterokinase configuration, providing three different reading frames at the multicloning site; Kmr | Novagen |
| pDM4 | A suicide vector with ori R6K sacB, Cmr | 40 |
| pLAFR3 | IncP cosmid vector; Tcr | 54 |
| pLAFR3II | pLAFR3 with bla inserted at the cos site | This study |
| pRK2013 | IncP Kmr Tra Rk2+repRK2 repE1 | 9 |
| pCMM1701 | pLAFR3I1 with hlyU ORF | This study |
| pUTKm1 | Tn5-based insertion delivery plasmid; Ampr | 23 |
a
Cmr, CHL resistance; Tcr, TET-resistance; Kmr, KAN resistance; Ampr, AMP resistance.