TABLE 2.
Primers used for the mutant construction
| Mutant strain | Primer and primer sequence used for mutation
|
Amplified or deleted (Δ) region/total ORF (bp) | |
|---|---|---|---|
| Name | Sequence (restriction enzyme)a | ||
| CMM1701 | Pive-1-1 | ggctccctatttttatttctcagc | |
| Pive-1-2 | aagcttcaccatatcttgatagatcgc (HindIII) | Δ481-1860/2,004 | |
| Pive-1-3 | aagcttaacgtcagcgaaagtgtcacc (HindIII) | ||
| Pive-1-4 | cgaaatcgtggtagctgttgacg | ||
| CMM1702 | Pive-2-1 | gaagatctcgatggcctaggtgtgttggg (BglII) | 12-631/900 |
| Pive-2-2 | gctctagacgccattgccaccaacacagc (XbaI) | ||
| CMM1703 | Pive-3-1 | gaagatcttctagtgattgtgaataccagc (BglII) | 456-993/1,161 |
| Pive-3-2 | gctctagaaacaactaagtagttattcacc (XbaI) | ||
| CMM1704 | Pive-4-1 | tggctcaggaagtaaaag | 120-618/726 |
| Pive-4-2 | gctctagatgtaaatgctgccaaatc (XbaI) | ||
| CMM1705 | Pive-5-1 | cgacatcggtggcccaaccatg | 378-1,170/1,593 |
| Pive-5-2 | gaagatctttgaaccagtaggccgccg (BamHI) | ||
| CMM1706 | Pive-6-1 | gctctagaccagcacgaaccattgcaac (XbaI) | 382-1,205/1,485 |
| Pive-6-2 | gaagatctcgctgatccctcaagctgatc (BglII) | ||
| CMM1707 | Pive-7-1 | ggcttcatttcatgaagccgc | |
| Pive-7-2 | aagctttccgaatagtctcacataggg (HindIII) | Δ34-384/393 | |
| Pive-7-3 | aagcttcaataccagttcccaaaaacc (HindIII) | ||
| Pive-7-4 | tggatgatgaatcaagttggtcc | ||
| CMM1708 | Pive-8-1 | atgcaatcgtaaactcacagc | |
| Pive-8-2 | aagcttggctttcatgtaatg (HindIII) | Δ10-165/231 | |
| Pive-8-3 | aagcttttagaaattcctgaacac (HindIII) | ||
| Pive-8-4 | gctcagggcttacactttctg | ||
| CMM1709 | Pive-9-1 | gctctagaactagccagcaactcttcac (XbaI) | 126-685/891 |
| Pive-9-2 | gaagatctacaatcgcttcatgacctga (BglII) | ||
| CMM1710 | Pive-10-1 | cgggatcctcgatatcgtcaactatag (BamHI) | |
| Pive-10-2 | cgggatccttttaagttcatgtgttgg (BamHI) | Δ13-291/297 | |
| Pive-10-3 | cgggatccgaataatgcttttgcgtg (BamHI) | ||
| Pive-10-4 | tacgtcggcactggcacctg | ||
| CMM1711 | Pive-11-1 | gaagatctgtaaaagttggcgttatggcg (BglII) | 97-710/810 |
| Pive-11-2 | gaagatcttgaacgttgtcttggcgagc (BglII) | ||
| CMM1712 | Pive-12-1 | gaagatctcaagaaaacaaccactctc (BglII) | 106-1,197/1,410 |
| Pive-12-2 | gaagatctatctaggttctctttggc (BglII) | ||
a
The restriction enzyme site is underlined.