Table 1.
Intragenic suppressors of YR1m4
| Mutation | No. of times isolated | Cell growth
|
|
|---|---|---|---|
| At 30°C | At 37°C | ||
| R149C | 13 | ++++ | ++++ |
| R149S | 5 | ++++ | ++++ |
| R149G | 4 | +++ | +++ |
| N152I | 1 | ++++ | +++ |
| R182L | 4 | ++++ | +++ |
| R182S | 3 | ++++ | + |
| R182C | 3 | +++ | + |
| G115R | 1 | +++ | +/− |
| N117Y | 2 | ++++ | +++ |
| YR1m4 | NA | + | − |
| yTFIIB | NA | +++++ | +++++ |
Listed are intragenic suppressor mutants of YR1m4, number of times they were isolated, and their ability to support cell growth at both 30°C and 37°C. The original data on cell growth are shown in Fig. 4. See text for further details. NA, not applicable.