FIG. 3.

Inhibition of invasion of A549 cells by dominate negative and constitutive forms of PI 3-K. Data are expressed as the percent invasion of transiently transfected A549 cells relative to untransfected A549 cells with the LT1 transfection reagent. LT1 cells were exposed to the LT1 polyamine transfection reagent without plasmid DNA. The cells were transfected with pSG5 (vector only), pSG5-p85 (dominant negative p85), pSG5-p110 (dominant negative p110), or p110CAAX (constitutively active form of p110) (30). They were also transfected with a GFP fusion (pEGFP-p85) or vector only (pEGFPC2) (20).