TABLE 1.

Effect of different inhibitors on the invasion of HEp-2 cells by GAS^a

Inhibitor (concn)	% Inhibition
None	0.0
Calphostin C (250 nM)	1.6
Bisindolylmaleimide (50 nM)	3.0
TPA (100 nM for 30 min)	3.8
TPA (100 nM for 24 h)	2.0
Rapamycin	3.6

^aThe cells were preincubated with the inhibitor for 30 min, and invasion was performed in the presence of inhibitor for 2 h. Percent invasion is calculated as follows: [(CFU_{no inhibitor} − CFU_inhibitor)/CFU_{no inhibitor}] × 100.