TABLE 1.
Bacterial strains used in this study
| Strain and original designationa | Serotype | LDCb | Invasivenessc | Relevant featuresd | Country of origin or source (reference)e |
|---|---|---|---|---|---|
| EIEC | |||||
| HN280 | O135 | − | + | Lac−, pINV (260 kb), pCRY (160 kb) | Somalia (24) |
| HN11 | O135 | − | − | Lac−, pINV (260 kb), pCRY (160 kb) | Somalia (24) |
| HN13 | O135 | − | + | Lac−, pINV (260 kb), pCRY (160 kb), pR (Cmr Hgr Smr Sur Tcr [100 kb]) | Somalia (24) |
| 4608 | O143 | − | + | Lac+, pINV (250 kb) | WRAIR (35) |
| 53638 | O144 | − | + | Lac−, pINV (250 kb), pCRY (140 kb) | WRAIR (35) |
| 13.80 | O124 | − | + | Lac− pINV (240 kb) | IPC (35) |
| 6.81 | O115 | − | + | Lac− pINV (250 kb), pCRY (160 kb) | IPC (35) |
| S. flexneri | |||||
| SFZM50 | 1b | − | + | SRL, pINV (220 kb), pR (Sur Smr [6.3 kb]), pCRY (4.1, 3.2, 2.7, 2.3, and 1.6 kb) | Somalia (5) |
| SFZM46 | 2a | − | + | SRL, pINV (220 kb), pR (Sur Smr [6.3 kb]), pCRY (4.1 and 3.2 kb) | Somalia (5) |
| YSH6000 | 2a | − | + | SRL, pINV (230 kb), pCRY (4.1 and 3.2 kb) | Japan (37) |
| SFZM49 | 3a | − | + | SRL, pINV (220 kb), pCOL (5.2 kb, Col+), pCRY (3.2 and 2.1 kb) | Somalia (5) |
| SFZM53 | 4 | − | + | SRL, pINV (220 kb), pR (Sur Smr [6.3 kb]), pCRY (7.0, 4.1, 3.2, 1.9, and 1.6 kb) | Somalia (5) |
| M90T | 5 | − | + | pINV (213 kb), pCRY (2.1 and 1.6 kb) | WRAIR (35) |
| SFZM43 | 6 | − | + | SRL, pINV (220 kb), pR (Sur [6 kb]), pCRY (4.1, 2.8, 2.7, and 1.9 kb) | Somalia (5) |
a
All the strains are designated by their original laboratory name.
b
LDC, lysine decarboxylase. Lysine decarboxylase activity has been tested by the method of Meng and Bennet (20).
c
Bacterial invasion of HeLa cells was measured as previously described (35).
d
SRL, Shigella resistance locus (Apr Cmr Spr Tcr) (41); pINV, invasivity plasmid; pCRY, cryptic plasmid; pR antibiotic resistance plasmid; pCOL, colicin-producing plasmid. Cm, chloramphenicol, Hg, hygromycin, Sm, streptomycin, Su, sulfonamide; Tc, tetracycline; Ap, ampicillin; Sp, spectinomycin.
e
WRAIR, Walter Reed Army Institute of Research; IPC, Institut Pasteur Collection.