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Analysis of the cad locus in S. flexneri
| Strain | Size (kb) of PCR product (primers)
|
|||||
|---|---|---|---|---|---|---|
| ytfa cadB (Y2-B2) | cadA (A1-A2) | cadB (B1-B3) | cadB cadC (B2-C2) | cadB IS1 (B2-I1) | IS1 cadB (I1-B2R) | |
| MG1655a | NDc | 2.1 | 1.7 | 2.5 | ND | ND |
| SFZM50 | 4.7 | NP | NP | 1.2 | 0.8 | 2.0 |
| SFZM46 | 4.7 | NP | NP | 1.2 | 0.8 | 2.0 |
| YSH6000 | 4.7 | NP | NP | 1.2 | 0.8 | 2.0 |
| SFZM49 | NPb | NP | NP | NP | ND | ND |
| SFZM53 | 4.7 | NP | NP | 1.2 | 0.8 | 2.0 |
| M90T | 4.7 | NP | NP | 1.2 | 0.8 | 2.0 |
| SFZM43 | NP | NP | NP | NP | ND | ND |
E. coli K-12, used as control.
NP, no PCR product.
ND, not done.
