FIG. 6.

Hierarchical clustering of hybridization signal intensity (gene expression) differences for pro- and anti-inflammatory cytokine probe sets. The gene expression observations from the single subject with three replicates were variance normalized and subjected to hierarchical clustering. The variation in gene expression for a given gene is expressed as the distance from the mean observation for that gene (S.D., standard deviations). Several genes were represented by more than one probe set on the Affymetrix U95aVer2 chip, and the different probe sets are identified by their Affymetrix identification numbers. CON, control unstimulated leukocytes; LPS, leukocytes exposed to LPS for 2 h prior to RNA harvest; SAC, leukocytes exposed to S. aureus Cowan for 2 h prior to RNA harvest; r, replicate number.